Allosteric Properties of a Pyruvate Kinase Isoenzyme from Rat Liver Cells in Culture
نویسندگان
چکیده
منابع مشابه
Allosteric properties of skeletal muscle pyruvate kinase.
The phenylalanine inhibition of skeletal muscle pyruvate kinase has been further studied. Even at high levels of the inhibitor the plots of reaction rates as a function of phosphoenolpyruvate concentration give hyperbolic curves; the inhibition by phenylalanine is of the mixed type. The enzyme exhibits a homotropic cooperative effect with respect to this inhibitor. This property is strongly dep...
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It is shown here that rat liver pyruvate dehydrogenase (PDH) kinase activator protein (KAP) catalyses ATP-dependent inactivation and [32P]phosphorylation of pig heart PDHE1 and of yeast (Saccharomyces cerevisiae) PDH complex devoid of PDH kinase activity, that fluorosulphonylbenzoyladenosine inactivates rat liver KAP and the intrinsic PDH kinase of rat liver PDH complex, and that KAP, like PDH ...
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The conversion of precancerous lesions to full-fledged cancers requires the affected cells to surpass certain rate-limiting steps. We recently showed that activation of HRAS proto-oncogene in urothelial cells of transgenic mice causes simple urothelial hyperplasia (SUH) which is persistent and whose transition to low-grade papillary urothelial carcinoma (UC) must undergo nodular urothelial hype...
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The properties ofpyruvate kinase from soybean ( Glycinemax L.) nodule cytosol were examind to determine what influence the N2 fixation process might have on this supposed key control enzyme. A crude enzyme preparation was prepared by chromatography of cytosol extract on a diethylaminoethyl-cellulose column. ATP and citrate at 5 mm concentrations inhibited pyruvate kinase 27 and 34%, respectivel...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43707-1